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S4 Biology: Cellular Respiration Paper 4 (Mixed Calculations)

S4 Biology: Cellular Respiration Paper 4 (Mixed Calculations)

  • 2026-11-03

S · 4

S4 Biology: Cellular Respiration Paper 4 (Mixed Calculations)

This closing paper puts ATP yield, mole conversions, respiratory quotient and efficiency together. Write the formula first, then substitute.

Quick answer 1. 36 ATP (2/2/32) | 2. 6 O2, 6 CO2, 6 H2O | 3. 2 ethanol + 2 CO2 | 4. lactate and oxygen debt | 5. 60 cm3 | 6. RQ 0.83, carbohydrate | 7. 38.3% | 8. +300% | 9. about 1.5 g | 10. 2 ATP, 18 times

01 Paper details (check answers afterwards)

Item Detail
Questions 10 questions
Suggested time 25 minutes
Difficulty mix Foundation 4 · Intermediate 3 · Challenge 2 · Error redo 1
Chapter Chapter: S4 Biology — Cellular Respiration
Level Challenge

02 Questions (10 items)

  1. FoundationHow many ATP are gained net from complete oxidation of one glucose molecule, and how much does each stage contribute?
  2. FoundationComplete oxidation of one glucose molecule needs how many oxygen molecules, and releases how many carbon dioxide and water molecules?
  3. FoundationWhat does yeast produce when it breaks down one glucose molecule without oxygen, and how many molecules of each?
  4. FoundationDuring hard exercise with too little oxygen, what does glucose become, and why does breathing stay heavy afterwards?
  5. IntermediateComplete oxidation of 0.45 g glucose releases what volume of carbon dioxide at room temperature? (molar volume 24 dm3, C6H12O6 = 180)
  6. IntermediateA tissue uses 6.0 mol of oxygen and releases 5.0 mol of carbon dioxide per minute. What is its respiratory quotient and what fuel dominates?
  7. IntermediateComplete oxidation of one glucose releases 2 870 kJ and each ATP stores 30.5 kJ. What is the storage efficiency?
  8. ChallengeAn athlete raises oxygen uptake from 0.25 dm3 to 1.00 dm3 per minute. By what percentage does it rise?
  9. Challenge200 cm3 of carbon dioxide is collected at room temperature from complete oxidation of glucose. What mass of glucose was used at least?
  10. Error redoA student claims anaerobic fermentation of one glucose nets 36 ATP. What is correct, and how many times larger is the aerobic yield?

03 Answers and one-line notes

# Answer One-line note
1 Net gain is 36 ATP: 2 from glycolysis, 2 from the Krebs cycle and 32 from oxidative phosphorylation. Substrate-level phosphorylation gives only four; the rest comes from hydrogen passing down the chain.
2 It needs 6 oxygen molecules and releases 6 carbon dioxide and 6 water molecules. Atoms must balance on both sides, so the coefficient stays at six, never twelve.
3 It produces 2 ethanol molecules and 2 carbon dioxide molecules. Alcoholic fermentation stops two steps after glycolysis, so most energy stays in the ethanol.
4 Glucose becomes lactate; extra oxygen is taken in afterwards to oxidise it, and that extra amount is the oxygen debt. Lactate is carried to the liver, which is why deep breathing continues after a sprint.
5 0.45 g is 0.0025 mol, so 0.0025 x 24 dm3 = 0.060 dm3, that is 60 cm3. Find moles from mass, multiply by molar volume, then convert dm3 into cm3.
6 RQ = 5.0 / 6.0 = 0.83, close to 1, so carbohydrate is the main fuel. Fat gives an RQ near 0.7, so closer to 1 means more carbohydrate.
7 36 x 30.5 = 1 098 kJ, and 1 098 / 2 870 = 38.3%. The rest leaves as heat, which is why exercise raises body temperature.
8 (1.00 – 0.25) / 0.25 x 100% = 300%, a rise of 300%. The rise is divided by the original value, not the new one.
9 200 / 24 000 = 0.00833 mol, so mass = 0.00833 x 180 = about 1.50 g. Each glucose gives six carbon dioxide molecules, and that ratio is already inside the mole conversion.
10 Only 2 ATP is correct; 36 / 2 = 18 times more aerobically, because hydrogen has no chain to run down without oxygen. Mixing the aerobic total with the glycolytic net gain is the commonest error.

04 What to do after marking

  • After marking, redo only the wrong items and write down which step went wrong (formula, unit, or misread number).
  • Repeat the same item two days later; move to the next paper only when both attempts are correct.
  • Parents only need the answer and note columns; there is no need to explain every item.

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